Vimentin Antikörper (CF®488A)

Produktnummer ABIN6934573

Dieser Anti-Vimentin Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von Vimentin in IF, FACS und T. Geeignet für Human, Schwein, Rind (Kuh), Huhn, Hund, Cat und Ziege.

Kurzübersicht für Vimentin Antikörper (CF®488A) (ABIN6934573)

Target: Vimentin (VIM)

Reaktivität: Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist konjugiert mit CF®488A

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Test (T)

Klon: VM452

Produktdetails anti-Vimentin Antikörper (CF®488A)

Spezifität: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human vimentin protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat cells, Sarcomas, Melanomas.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: VIM

Molekulargewicht: 57-60kDa

Gen-ID: 7431

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose